DFCI

Chargeback Fees for MBCF Services

Updated: 11/02/2010

DNA Synthesis

Mass Spectrometry

DNA and Protein Database searches

DNA Sequencing

Protein Sequencing

BIAcore protein ligand assays

Peptide Synthesis

Amino Acid Analysis

Molecular Biology Consultation

Pricing Breakdown:

"Inside the Institute/CFAR" = pricing schedule if your lab is inside DFCI or your lab is a member of
the Center for AIDS Research grant. (If you are unsure, then likely you are outside the Institute)
"Outside the Institute" = Basically, if you cannot pay for the service on a DFCI cost center number
you are "Outside the Institute"


DNA Synthesis

 

Inside the Institute /CFAR

Outside the Institute

MBCF Sequencing Primer*

FREE* !!

FREE* !!

40 nanomole /purified/ quantitated

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0.2 micromole /unpurified

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1.0 micromole /unpurified

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biotin, aminolink, etc

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*MBCF Sequencing Primer price is in effect if the oligo goes directly from synthesis to sequencing without researcher intervention.
If you come by and ask for an aliquot, the price may revert to $0.50 per nucleotide.
Integration into a sequencing project and non interference by the researcher is key to free sequencing primers.


DNA Sequencing

Pricing is per sequencing run. Read Sequencing Guidelines for explanation of different services.

 

Inside the Institute /CFAR

Outside the Institute

Fast Track Primer Added

< 16 Samples

$8.00

$8.00

16-64 Samples

$6.00

$6.00

> 64 Samples

$4.00

$6.00

Fast Track Primer NOT Added

$8.00

$10.00

Difficult Template

$12.00

$15.00

96 format high volume

$6.00 or less e-mail for quote

$6.00 or lesse-mail for quote

Sequencher contig building

$25.00 per complete build

$50.00 per complete build

Sequencher program installed

free within institute network

unavailable. see Gene-Codes

All data are deposited into your MBCF account and archived. Current archives go back to 1992. Instant access archiving goes back to November 2005.
We guarantee at least 1,000 nucleotides and same day delivery.


Genotyping, High Volume Sequencing

 

Inside the Institute /CFAR

Outside the Institute

Genotyping

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Sequencing 96 plate

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Peptide Synthesis

All peptides are now HPLC purified, then analyzed by mass analysis and analytical HPLC.

 

Inside the Institute /CFAR

Outside the Institute

base charge (HPLC purification/analysis)

$195.00

$295.00

charge per amino acid

$20.00

$30.00

Extras:

phospho Tyrosine

$30.00 additional

$50.00 additional

phospho Serine

$30.00 additional

$50.00 additional

phospho Threonine

$100.00 additional

$200.00 additional

biotinylated

$75.00 additional

$100.00 additional

Multiple Antigenic Peptide (MAP) 

$125.00 additional

$175.00 additional

Conjugation to KLH

$100.00 additional

$100.00 additional

other modifications

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call for quote

EXAMPLE: Peptide ordered within DFCI. 20 amino acids in length with one serine phosphorylated.
$195.00+(20x$20.00)+$30.00=$625.00 for an HPLC purified peptide. 20-50mg typical yield.
 


Mass Spectrometry

 

 

Inside the Institute /CFAR

Outside the Institute

mass analysis

$25.00

$50.00

tryptic digest and mass analysis by MALDI-TOF

$150.00 first sample, $50.00 additional samples

$300.00 first sample, $100.00 addtitional samples

tryptic digest and mass analysis by MALDI-TOF + LC-MS/MS

$200.00 first sample, $100.00 additional samples

$400.00 first sample, $200.00 addtitional samples

mass analysis of pre-digested sample by MALDI-TOF

$50.00

$100.00

mass analysis of pre-digested sample by MALDI-TOF + LC-MS/MS

$100.00

$200.00

additional methods

call for quote

call for quote

1 hour user operated session

$50.00

$50.00

instrument operation tutorial (1-3 researchers)

$200.00

$200.00

If you submit proteins for tryptic digestion and identification, you may submit one blank gel slice and one standard protein gel slice at no cost.
Also various means of pricing projects depends on if the researcher is trained to use the instrument.
The mass analysis service is currently expanding.
Come by and discuss what kind of problem you have that might be solved by accurate, sensitive mass analysis.
 


Protein Sequencing

N-terminal sequencing by Edman degradation.


 

 

Inside the Institute /CFAR

Outside the Institute

start-up

no charge

no charge

charge per cycle

$15.00

$20.00

tryptic digestion

$50.00

$100.00

HPLC peptide mapping

$50.00

$100.00

additional methods

call for quote

call for quote


 


DNA and Protein Database searches

Entire current database.


 

 

Inside the Institute /CFAR

Outside the Institute

first search

$25.00

$35.00

each additional search

$2.00

$3.00


BIAcore protein ligand assays

 

 

Inside the Institute /CFAR

Outside the Institute

User operated session

$150 /day

$150 /day

Tutorial with Getting Started Kit

$340.00

$340.00

4 cell dextran chip

$175.00

$175.00

other chips

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Pricing can depend on the amount of training required before the researcher gets results.


Amino Acid Analysis

Free with each synthetic peptide

 

Inside the Institute /CFAR

Outside the Institute

For concentration determination

 $25.00

$35.00


Molecular Biology Consultation

 

Inside the Institute /CFAR

Outside the Institute

first hour

free of charge

$300.00

each additional hour

$200.00

call for quote